The role of 3D chromatin architecture in regulation of gene expression and cell identity
Regulation of gene expression: Multiple layers of complexity
and Histone Variants
Somatic Cell Reprogramming
Complex interplay between TFs and epigenetic layers
To understand the complex functional interplay between transcription factors and 3D chromatin architecture and the impact on cell fate decisions, such as reprogramming, differentiation or tumorigenesis.
We use high-throughput sequencing techniques such as 4C-Seq, HiC, HiChIP, ChIP-seq and RNA-seq and integrative computational analysis to dissect regulatory mechanisms of cell type-specific gene expression programs. We also employ advanced CRISPR/(d)Cas9-based genetic and epigenetic approaches for functional validation.
Genomic distribution of Nanog-interacting regions - The Nanog locus interacts with many chromatin domains along its own chromosome
· Delineating the mechanisms of cell fate inheritance after cell division.
· Dissecting the nature, role and major driving forces of 3D chromatin reorganization during cell fate transitions.
· Defining critical proteins and regulatory elements that control epigenetic stability of genomic imprinting.
© 2016 Apostolou Lab @ Weill Cornell Medical College