The role of 3D chromatin architecture in regulation of gene expression and cell identity
Regulation of gene expression: Multiple layers of complexity
and Histone Variants
We use high-throughput sequencing techniques such as 4C-Seq, HiC, HiChIP, ChIP-seq and RNA-seq and integrative computational analysis to dissect regulatory mechanisms of cell type-specific gene expression programs. We also employ advanced CRISPR/(d)Cas9-based genetic and epigenetic approaches for functional validation.
· Delineating the mechanisms of cell fate inheritance after cell division.
· Dissecting the nature, role and major driving forces of 3D chromatin reorganization during cell fate transitions.
· Defining critical proteins and regulatory elements that control epigenetic stability of genomic imprinting.
© 2016 Apostolou Lab @ Weill Cornell Medical College