RESEARCH

The role of 3D chromatin architecture in regulation of gene expression and cell identity

Regulation of gene expression: Multiple layers of complexity

Signal Transduction

Pathways

Transcription Factors

and Co-factors

DNA Methylation

Chromatin Modifications

and Histone Variants

Subnuclear Localization

and Long-Range

Interactions

 

Focus

Somatic Cell Reprogramming​

Complex interplay between TFs and epigenetic layers

To understand the complex functional interplay between transcription factors and 3D chromatin architecture and the impact on cell fate decisions, such as reprogramming, differentiation or tumorigenesis.

 

Approach

We use high-throughput sequencing techniques such as 4C-Seq, HiC, HiChIP, ChIP-seq and RNA-seq and integrative computational analysis to dissect regulatory mechanisms of cell type-specific gene expression programs. We also employ advanced CRISPR/(d)Cas9-based genetic and epigenetic approaches for functional validation.

 

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Projects/Directions

·       Delineating the mechanisms of cell fate inheritance after cell division.

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·       Dissecting the nature, role and major driving forces of 3D chromatin reorganization during cell fate transitions.

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·       Defining critical proteins and regulatory elements that control epigenetic stability of genomic imprinting.

 

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© 2016 Apostolou Lab @ Weill Cornell Medical College

Model for Nanog's interactome changes during reprogramming